ESPID Education. de Lauzanne A. 06/07/11; 7726
Dr. Agathe de Lauzanne
Dr. Agathe de Lauzanne

This content is available to ESPID members only

Click here for more information or if you are considering becoming an ESPID member.

Discussion Forum (0)
Rate & Comment (0)
QuantiFERON®-TB Gold in-tube performance for tuberculosis infection diagnosis in 0-5 years of age children.

Camille Debord1, Agathe De Lauzanne2, Valérie Guérin-El Khourouj1, Nadège Gourgouillon1, Béatrice Pédron1, Joel Gaudelus3, Albert Faye2, and Ghislaine Sterkers1.

1 Laboratory of Immunology, Robert Debré Hospital, AP-HP, Paris VII University, Paris-France.
2 Department of Pediatrics, Robert Debré Hospital, AP-HP, Paris VII University, Paris-France.
3 Department of Pediatrics, Jean Verdier Hospital, AP-HP, Paris VII University, Paris-France.

Background: Interferon-γ-Release Assays (IGRAs) are increasingly used for the diagnosis of tuberculosis infection (TB) in adults. Unknown performance in infants and controversial rate of indeterminate results in <5 years old children limit their pediatric usage.
Method: immunocompetent children (n=82, 0.10 to 5.6 years of-aged; median: 1.8) referred for suspected TB infection or contact with TB and sequentially evaluated for QuantiFERON®-TB Gold In-Tube (QF-TB-IT) reactivity were included for QF-TB-IT performance evaluation.
Results: BCG vaccination rate at birth was high (91%). Active TB was diagnosed in 15 children; 10 had latent TB i.e. recent TB contact and positive Tuberculin-Skin Test (TST); 16 had recent contact with TB but negative TST (healthy contact); the 41 remaining children, with TB excluded, were used as a referent population. The rate of QF-TB-IT indeterminate was 5% in active TB, latent TB and healthy contact children (0% in <2 years; n=23) while 22% in referent population with irrelevant diseases. QF-TB-IT sensitivity and specificity, as determined by positivity in active TB and negativity in controls were 86% and 100% respectively (67% and 100% in <2 years). Nearly half of the children with latent TB (4/9) had QF-TB-IT positivity (2 of 4 were <2 years). Finally, concordance between TST and QF-TB-IT results was high only when considering TST negativity <10mm and positivity >15mm induration diameter respectively.
Conclusion: QF-TB-IT sensitivity and specificity were high and the rate of indeterminate results was low in <0-5 years children with TB or TB contact. QF-TB-IT appears therefore a promising tool for TB diagnosis in young immunocompetent children.
Code of conduct/disclaimer available in General Terms & Conditions

By clicking “Accept Terms & all Cookies” or by continuing to browse, you agree to the storing of third-party cookies on your device to enhance your user experience and agree to the user terms and conditions of this learning management system (LMS).

Cookie Settings
Accept Terms & all Cookies